Metabolomics

Responsible: Pierre Pétriacq

Metabolome platform provides support for research in biology, environmental physiology, functional genomics and genetics in the context of regional, national and international collaborations. It is also used in the analysis of products derived from plants and other organisms.

The platform provides:

  • Quantification of amino acids by UHPLC-fluorimetry
  • Quantification of major polar metabolites by 1H-NMR of polar extracts
  • Acquisition and analysis of metabolomic fingerprints by 1H-NMR of polar extracts
  • Acquisition and analysis of metabolomic fingerprints by LC-Orbitrap-MS of semi-polar extracts
  • Acquisition of spectra for the measurement of 13C isotopic enrichments on fractions, for metabolic flux measurement

Analytical Equipements

  • One LC-QqTOF-MS system for metabolic profiling,
  • One UHPLC-UHRMS LTQ-Orbitrap system,
  • One LC-Qtrap-MS systems,
  • UPLC system for the separation and quantification of amino acids (inverse phase and fluorimetry detection)

Key publications

  • Ghina Hajjar, Millena C. Barros Santos, Justine Bertrand-Michel, Cécile Canlet, Florence Castelli, Nicolas Creusot, Sylvain Dechaumet, Binta Diémé, Franck Giacomoni, Patrick Giraudeau, Yann Guitton, Etienne Thévenot, Marie Tremblay-Franco, Christophe Junot, Fabien Jourdan, François Fenaille, Blandine Comte, Pierre Pétriacq and Estelle Pujos-Guillot (2023). Scaling-up metabolomics: Current state and perspectives. TrAC Trends in Analytical Chemistry [link to the publication]
  • Gan ShaGuillaume Decros, Thomas Dussarrat, Pierre Baldet, Cédric Cassan, Cécile Cabasson, Martine Dieuaide‐Noubhani, Alice Destailleur, Amélie Flandin, Sylvain Prigent, Kentaro Mori, Sophie Colombié, Joana Jorly, Yves Gibon, Bertrand Beauvoit and Pierre Pétriacq (2023). Enzyme‐based kinetic modelling of ASC–GSH cycle during tomato fruit development reveals the importance of reducing power and ROS availability. New Phytologist [link to the publication]
  • Poucet T., Beauvoit B., Gonzalez-Moro M., Cabasson C., Pétriacq P., Flandin A., Gibon Y., Marino D. and Dieuaide-Noubhani M. (2022). Impaired cell growth in ammonium stress explained by modelling the energy cost of vacuole expansion in tomato leaves. The Plant Journal [link to the publication]
  • Dussarrat T, Prigent S, Latorre C, Bernillon S, Flandin A, Díaz FP, Cassan C, Van Delft P, Jacob D, Varala K, Joubes J, Gibon Y, Rolin D, Gutiérrez RA and Pétriacq P. (2022). Predictive metabolomics of multiple Atacama plant species unveils a core set of generic metabolites for extreme climate resilience. New Phytologist [link to the publication]
  • Nicolas Creusot, Betty Chaumet, Mélissa Eon, Nicolas Mazzella, Aurélie Moreira and Soizic Morin (2022). Metabolomics insight into the influence of environmental factors in responses of freshwater biofilms to the model herbicide diuron. Environmental Science and Pollution Research [link to the publication]