High-throughput biochemical phenotyping

Responsible: Yves Gibon

Metabolome platform provides support for research in biology, environmental physiology, functional genomics and genetics in the context of regional, national and international collaborations. It is also used in the analysis of products derived from plants and other organisms.

The platform provides:

  • Automated analyses on microplates: total proteins, starch, sucrose, glucose, fructose, malate, fumarate (under proviso), citrate, total free amino acids, glutamate, proline, chlorophylls a and b (from one extraction)
  • Automated measurement of central-metabolism enzymes (glycolysis, TCA cycle, Calvin-pentose phosphates, sucrose metabolism, nitrogen assimilation)

Analytical Equipement

  • Integrated pipetting robots,
  • Temperature control,
  • 11 UV/visible microplate readers and one multifunction microplate reader,
  • A dedicated server for data storage and PC stations with a LIMS.

Key publications

  • Sophie Colombié, Sylvain Prigent, Cédric Cassan, Ghislaine Hilbert‐Masson, Christel Renaud, Emilia Dell’Aversana, Petronia Carillo, Annick Moing, Chloé Beaumont, Bertrand Beauvoit, Tim McCubbin, Lars Keld Nielsen and Yves Gibon (2023). Comparative constraint‐based modelling of fruit development across species highlights nitrogen metabolism in the growth‐defence trade‐off. The Plant Journal [link to the publication] [link to the data used in the publication]
  • Céline Bournonville, Kentaro Mori, Paul Deslous, Guillaume Decros, Tim Blomeier, Jean-Philippe Mauxion, Joana Jorly, Stéphanie Gadin, Cédric Cassan, Mickael Maucourt, Daniel Just, Cécile Brès, Christophe Rothan, Carine Ferrand, Lucie Fernandez-Lochu, Laure Bataille, Kenji Miura, Laure Beven, Matias D Zurbriggen, Pierre Pétriacq, Yves Gibon and Pierre Baldet (2023). Blue light promotes ascorbate synthesis by deactivating the PAS/LOV photoreceptor that inhibits GDP-L-galactose phosphorylase. The Plant Cell [link to the publication]
  • Clarisse Cornebise, Maude Perus, François Hermetet, Josep Valls-Fonayet, Tristan Richard, Virginie Aires and Dominique Delmas (2023). Red Wine Extract Prevents Oxidative Stress and Inflammation in ARPE-19 Retinal Cells. Cells [link to the publication]
  • Hendrik Poorter, Xinyou Yin, Nouf Alyami, Yves Gibon and Thijs L. Pons (2022). MetaPhenomics: quantifying the many ways plants respond to their abiotic environment, using light intensity as an example. Plant and Soil [link to the publication]
  • Nadia A Campos, Sophie Colombié, Annick Moing, Cedric Cassan, Delphine Amah, Rony Swennen, Yves Gibon and Sebastien C Carpentier (2022). From fruit growth to ripening in plantain: a careful balance between carbohydrate synthesis and breakdown. Journal of experimental botany [link to the publication]