Responsible: Laetitia Fouillen
The platform brings together instrumentations and expertise dedicated to the analysis of the lipidome and the study of lipids in their diversity. It is dedicated to the study of many organisms, from plants to animals and yeast.
Metabolome platform provides support for research in biology, environmental physiology, functional genomics and genetics in the context of regional, national and international collaborations. It is also used in the analysis of products derived from plants and other organisms. It is mainly used for the establishment and interpretation of lipidomic profiles, identification and structural analysis of lipids and functional analysis of lipid metabolism.
The platform provides identification, characterization or quantification (absolute or relative) of lipid:
– Quantification by densitometry of class of lipids
– Identification and quantification of fatty acid (long chain, hydroxylated…) composition, waxes, cutines and suberins components
– Relative quantification of molecular species of glycerolipids : phospholipid: (PC, PE, PG, PS), anionic phospholipid (PA, PS, PI, PIP2, PIP3), neutral lipid (TAG, DAG, steryl ester…) and galactolipid (MGDG, DGDG, SQDG).
– Regiolocalization for TAG molecules
– Quantification of Alkyl HydroxyCinnamates (alkyl ferulate, alkyl coumarate and alkyl caffeate)
We adapted the lipid extraction and analyses to the desired materials.
Analytical Equipement
- Two GC-FID systems (Agilent) equipped with autosamplers and data processing stations,
- Two GC-MS systems equipped with an autosampler, an Agilent mass detector and a data processing station.
- Two LC-Qtrap-MS systems
- One HPTLC quantitative lipid analyzer constituted by autosamplers (Camag Linomat V), Automatic chamber (ADC2, Camag) and UV-visible TLC scanner (Camag Scanner 3)
Key publications
- Genva M., Fougere L., Bahammou D., Mongrand S., Boutte Y. and Fouillen L. (2024). A global LC-MS2-based methodology to identify and quantify anionic phospholipids in plant samples. Plant J [link to the publication]
- Gan Sha, Peng Sun, Xiaojing Kong, Xinyu Han, Qiping Sun, Laetitia Fouillen, Juan Zhao, Yun Li, Lei Yang, Yin Wang, Qiuwen Gong, Yaru Zhou, Wenqing Zhou, Rashmi Jain, Jie Gao, Renliang Huang, Xiaoyang Chen, Lu Zheng, Wanying Zhang, Ziting Qin, Qi Zhou, Qingdong Zeng, Kabin Xie, Jiandi Xu, Tsan-Yu Chiu, Liang Guo, Jenny C. Mortimer, Yohann Boutté, Qiang Li, Zhensheng Kang, Pamela C. Ronald and Guotian Li (2023). Genome editing of a rice CDP-DAG synthase confers multipathogen resistance. Nature [link to the publication]
- M. B. F. Steentjes, A. L. Herrera Valderrama, L. Fouillen, D. Bahammou, T. Leisen, I. Albert, T. Nurnberger, M. Hahn, S. Mongrand, O. E. Scholten and J. A. L. van Kan (2022). Cytotoxic activity of Nep1-like proteins on monocots. New Phytologist [link to the publication]
- Mamode Cassim Adiilah, Navon Yotam, Gao Yu, Decossas Marion, Fouillen Laetitia, Grélard Axelle, Nagano Minoru, Lambert Olivier, Bahammou Delphine, van Delft Pierre, Maneta-Peyret Lilly, Simon-Plas Françoise, Heux Laurent, Jean Bruno, Fragneto Giovanna, Mortimer Jenny C., Deleu Magali, Lins Laurence and Mongrand Sébastien (2021). Biophysical analysis of the plant-specific GIPC sphingolipids reveals multiple modes of membrane regulation. The Journal of biological chemistry [link to the publication]
- Garot E., Dussert S., Domergue F., Joet T., Fock-Bastide I., Combes M. C. and Lashermes P. (2021). Multi-Approach Analysis Reveals Local Adaptation in a Widespread Forest Tree of Reunion Island.. Plant Cell Physiol [link to the publication]